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Stimulus
Enzymes increase the rate of biochemical reactions by lowering the activation energy. The Michaelis-Menten model describes how reaction velocity varies with substrate concentration, approaching a maximum rate (Vmax) as the enzyme becomes saturated. The Michaelis constant (Km) represents the substrate concentration at which the reaction proceeds at half of Vmax.
| [S] (mM) | Reaction rate (μmol/min) |
|---|---|
| 0.5 | 12 |
| 1.0 | 22 |
| 2.0 | 38 |
| 4.0 | 56 |
| 8.0 | 71 |
| 16.0 | 82 |
Answer the following questions according to only the information provided.
Question 1. At which substrate concentration does the reaction reach approximately half its maximum velocity?
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